Student Sheet 13 – Effects of sugar on root explants


This protocol allows students to examine the growth of root explants of a 7 day period. It is a demanding technique, suitable for post-16 students with good lab skills, and some previous knowledge of aseptic technique would be an advantage.

For general plant tissue culture techniques in the school lab, see our improved and updated guide to Micropropagation and Tissue Culture and ‘Introducing STEM Careers – Conserving endangered species at Kew’, which puts the cauliflower cloning practical into a STEM careers context.

However, this protocol can be used by students for individual investigations into variables affecting root growth, and to consider the difference between the root tip and the mid-root explants.

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